diff --git a/.changes/next-release/bugfix-AmazonDynamoDB-1db6c7e.json b/.changes/next-release/bugfix-AmazonDynamoDB-1db6c7e.json
new file mode 100644
index 000000000000..e3c7c53e0746
--- /dev/null
+++ b/.changes/next-release/bugfix-AmazonDynamoDB-1db6c7e.json
@@ -0,0 +1,6 @@
+{
+    "type": "bugfix",
+    "category": "Amazon DynamoDB",
+    "description": "Fixed DurationAttributeConverter negative fractional duration serialization and parsing. Values like Duration.ofMillis(-1) now serialize as -0.001000000 and round trip correctly. Fixes [#7060](https://github.com/aws/aws-sdk-java-v2/issues/7060).",
+    "contributor": "Arnab Nandy"
+}
diff --git a/services-custom/dynamodb-enhanced/src/main/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverter.java b/services-custom/dynamodb-enhanced/src/main/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverter.java
index a135dba1416e..b0abd94ae005 100644
--- a/services-custom/dynamodb-enhanced/src/main/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverter.java
+++ b/services-custom/dynamodb-enhanced/src/main/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverter.java
@@ -79,9 +79,16 @@ public AttributeValueType attributeValueType() {
 
     @Override
     public AttributeValue transformFrom(Duration input) {
+        long seconds = input.getSeconds();
+        int nanos = input.getNano();
+        String value = seconds + (nanos == 0 ? "" : "." + padLeft(9, nanos));
+
+        if (input.isNegative() && nanos != 0) {
+            value = "-" + Math.abs(seconds + 1) + "." + padLeft(9, 1_000_000_000 - nanos);
+        }
+
         return AttributeValue.builder()
-                             .n(input.getSeconds() +
-                                (input.getNano() == 0 ? "" : "." + padLeft(9, input.getNano())))
+                             .n(value)
                              .build();
     }
 
@@ -103,10 +110,11 @@ private Visitor() {
         public Duration convertNumber(String value) {
             String[] splitOnDecimal = ConverterUtils.splitNumberOnDecimal(value);
 
+            boolean isNegative = value.startsWith("-");
             long seconds = Long.parseLong(splitOnDecimal[0]);
             int nanoAdjustment = Integer.parseInt(padRight(splitOnDecimal[1]));
 
-            if (seconds < 0) {
+            if (isNegative) {
                 nanoAdjustment = -nanoAdjustment;
             }
 
diff --git a/services-custom/dynamodb-enhanced/src/test/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverterTest.java b/services-custom/dynamodb-enhanced/src/test/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverterTest.java
index 477273721b3c..289ccabf0e1a 100644
--- a/services-custom/dynamodb-enhanced/src/test/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverterTest.java
+++ b/services-custom/dynamodb-enhanced/src/test/java/software/amazon/awssdk/enhanced/dynamodb/internal/converter/attribute/DurationAttributeConverterTest.java
@@ -61,6 +61,14 @@ void testConvertTo_NoPadding(String value, Duration expected) {
         assertThat(converted).isEqualByComparingTo(expected);
     }
 
+    @ParameterizedTest
+    @MethodSource("roundTripDurations")
+    void roundTrip(Duration duration) {
+        Duration converted = converter.transformTo(converter.transformFrom(duration));
+
+        assertThat(converted).isEqualByComparingTo(duration);
+    }
+
     static Stream<Arguments> noPadding() {
         return Stream.of(
             Arguments.of("0.123456789", Duration.ofNanos(123_456_789)),
@@ -74,7 +82,10 @@ static Stream<Arguments> noPadding() {
             Arguments.of("0.1",         Duration.ofMillis(100)),
             Arguments.of("0.001", Duration.ofMillis(1)),
             Arguments.of("0.000001", Duration.of(1, MICROS)),
-            Arguments.of("0.001", Duration.ofMillis(1))
+            Arguments.of("0.001", Duration.ofMillis(1)),
+            Arguments.of("-0.1", Duration.ofMillis(-100)),
+            Arguments.of("-0.001", Duration.ofMillis(-1)),
+            Arguments.of("-0.000001", Duration.of(-1, MICROS))
         );
     }
 
@@ -107,7 +118,22 @@ static Stream<Arguments> durations() {
             Arguments.of("9", Duration.ofSeconds(9)),
             Arguments.of("-9", Duration.ofSeconds(-9)),
             Arguments.of("0.001000000", Duration.ofMillis(1)),
-            Arguments.of("0.000000001", Duration.ofNanos(1)));
+            Arguments.of("0.000000001", Duration.ofNanos(1)),
+            Arguments.of("-0.001000000", Duration.ofMillis(-1)),
+            Arguments.of("-0.000000001", Duration.ofNanos(-1)),
+            Arguments.of("-1.234567890", Duration.ofNanos(-1_234_567_890)));
+    }
+
+    static Stream<Duration> roundTripDurations() {
+        return Stream.of(
+            Duration.ZERO,
+            Duration.ofNanos(1),
+            Duration.ofMillis(1),
+            Duration.ofSeconds(1, 234_567_890),
+            Duration.ofSeconds(-9),
+            Duration.ofMillis(-1),
+            Duration.ofNanos(-1),
+            Duration.ofNanos(-1_234_567_890));
     }
 
-}
\ No newline at end of file
+}